Gene description for PDCD6IP
Gene name programmed cell death 6 interacting protein
Gene symbol PDCD6IP
Other names/aliases AIP1
ALIX
DRIP4
HP95
MCPH29
Species Homo sapiens
 Experiment description of studies that identified PDCD6IP in Embryonic kidney cells

1
Experiment ID787
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID788
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - Fraction 4
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID789
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - Fraction 5
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Density gradient centrifugation
Flotation density1.07-1.13 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID790
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - S. salar genomic DNA stimulated
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID791
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - E. coli genomic DNA stimulated
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID792
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - condition medium collected after 5 min with S. salar genomic DNA stimulation
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID793
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - condition medium collected after 45 min without S.salar genomic DNA stimulation
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

8
Experiment ID794
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - condition medium collected after 45 min with S. salar genomic DNA stimulation
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

9
Experiment ID795
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - condition medium collected after 60 min
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

10
Experiment ID796
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - on ice with S. salar genomic DNA stimulation
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

11
Experiment ID797
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - DNase I treated
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

12
Experiment ID798
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - DNase I treated with S. salar genomic DNA stimulation
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

13
Experiment ID799
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

14
Experiment ID800
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - ODN 2137PS stimulated
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

15
Experiment ID801
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 29953723   
OrganismHomo sapiens
Experiment descriptionStimulation of exosome release by extracellular DNA is conserved across multiple cell types
AuthorsIliev D, Strandskog G, Nepal A, Aspar A, Olsen R, Jøsen J, Wolfson D, Ahluwalia BS, Handzhiyski J, Mironova R.
Journal name FEBS J
Publication year2018
SampleEmbryonic kidney cells
Sample nameHEK293T - ODN 2137PS stimulated
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

16
Experiment ID1659
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

17
Experiment ID1780
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35932288   
OrganismHomo sapiens
Experiment descriptionSpecific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides
AuthorsZhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G.
Journal name J Extracell Vesicles
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

18
Experiment ID1781
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35932288   
OrganismHomo sapiens
Experiment descriptionSpecific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides
AuthorsZhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G.
Journal name J Extracell Vesicles
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

19
Experiment ID1782
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35932288   
OrganismHomo sapiens
Experiment descriptionSpecific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides
AuthorsZhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G.
Journal name J Extracell Vesicles
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

20
Experiment ID1783
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 35932288   
OrganismHomo sapiens
Experiment descriptionSpecific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides
AuthorsZhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G.
Journal name J Extracell Vesicles
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

21
Experiment ID1925
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36214496   
OrganismHomo sapiens
Experiment descriptionContaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA
AuthorsMcCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ.
Journal name J Extracell Vesicles
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

22
Experiment ID1926
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36214496   
OrganismHomo sapiens
Experiment descriptionContaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA
AuthorsMcCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ.
Journal name J Extracell Vesicles
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

23
Experiment ID1932
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36214496   
OrganismHomo sapiens
Experiment descriptionContaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA
AuthorsMcCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ.
Journal name J Extracell Vesicles
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293 - fraction 3-4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

24
Experiment ID2010
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36417867   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles carrying HIV-1 Nef induce long-term hyperreactivity of myeloid cells
AuthorsDubrovsky L, Brichacek B, Prashant NM, Pushkarsky T, Mukhamedova N, Fleetwood AJ, Xu Y, Dragoljevic D, Fitzgerald M, Horvath A, Murphy AJ, Sviridov D, Bukrinsky MI.
Journal name Cell Rep
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

25
Experiment ID2011
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36417867   
OrganismHomo sapiens
Experiment descriptionExtracellular vesicles carrying HIV-1 Nef induce long-term hyperreactivity of myeloid cells
AuthorsDubrovsky L, Brichacek B, Prashant NM, Pushkarsky T, Mukhamedova N, Fleetwood AJ, Xu Y, Dragoljevic D, Fitzgerald M, Horvath A, Murphy AJ, Sviridov D, Bukrinsky MI.
Journal name Cell Rep
Publication year2022
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

26
Experiment ID3250
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34545097   
OrganismHomo sapiens
Experiment descriptionExosome-mediated stable epigenetic repression of HIV-1
AuthorsShrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV.
Journal name Nat Commun
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

27
Experiment ID3251
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34545097   
OrganismHomo sapiens
Experiment descriptionExosome-mediated stable epigenetic repression of HIV-1
AuthorsShrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV.
Journal name Nat Commun
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-qPCR
EV-TRACK -

28
Experiment ID3252
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34545097   
OrganismHomo sapiens
Experiment descriptionExosome-mediated stable epigenetic repression of HIV-1
AuthorsShrivastava S, Ray RM, Holguin L, Echavarria L, Grepo N, Scott TA, Burnett J, Morris KV.
Journal name Nat Commun
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Filtration
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyWestern blotting
RT-qPCR
EV-TRACK -

29
Experiment ID1408
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36706192   
OrganismHomo sapiens
Experiment descriptionDisplaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
AuthorsChoi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q.
Journal name Sci Adv
Publication year2023
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

30
Experiment ID1413
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36706192   
OrganismHomo sapiens
Experiment descriptionDisplaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
AuthorsChoi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q.
Journal name Sci Adv
Publication year2023
SampleEmbryonic kidney cells
Sample nameHEK293T - Fraction 5
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density1.151 g/mL
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

31
Experiment ID1659
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

32
Experiment ID1671
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

33
Experiment ID1671
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

34
Experiment ID1672
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

35
Experiment ID1672
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleEmbryonic kidney cells
Sample nameHEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

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